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Mini Vertical Electrophoresis Cell Tank Kit

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Additional Information

The Important Uses of Transfer Membranes Infographic

Feb 03, 2022 NuSep Inc.

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Nitrocellulose

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Nitrocellulose membranes are pure nitrocellulose media that has high binding capacity for proteins. These membranes are stronger and more durable than other nitrocellulose membranes, and will not rip, tear, or crack during transfers. The nature of this material yields sharper images with little or no distortion.

Features

The 0.45 µm pore size is recommended for most analytical blotting, including protein, ssDNA, and RNA transfers.

The 0.2 µm pore size is optimized for transferring nucleic acids and low molecular–weight proteins: the smaller pore size minimizes protein sample loss due to transfer through the membrane

➤ Compatible with all standard immunoblotting and nucleic acid detection methods

➤ Provides superior binding capacity without background interference

➤ Pore size produces excellent signal-to-noise blotting results

■ Transfer of protein from SDS-PAGE gels to membrane in preparation for western blotting

■ Transfer of a wide range of protein molecular weights and nucleic acids >500 bp (0.45 µm) / <500 bp (0.2 µm)

■ Transfer of nucleic acids in preparation for northern or Southern blotting

■ Immunodetection of target proteins and probe hybridization of nucleic acids

DBM-NIM-02 Transfer Membrane NC 0.45µm (8cm x 8cm) Pk15

DBM-NIM-03 Transfer Membrane NC 0.2µm (8cm x 8cm) Pk15

DBM-NIM-04 Transfer Membrane NC 0.45µm (7.9cm x 10.5cm) Pk15

DBM-NIM-05 Transfer Membrane NC 0.22µm (30cm x 3.5m) 1 roll/box

DBM-NIM-06 Transfer Membrane NC 0.45µm (30cm x 3.5m) 1 roll/box

RB-500 Transfer Membrane NC 0.45µm (3cm x 8.5cm) Pk10

RB-600 Transfer Membrane NC 0.22µm (7cm x 8.4cm) Pk10

RB-700 Transfer Membrane NC 0.45µm (7cm x 8.4cm) Pk10

PVDF

PVDF Membranes are ideal for chemiluminescent and colorimetric western blots: it retains target protein very strongly during protein transfer but also reduces nonspecific protein binding that can obscure high-sensitivity detection.

Features

➤ Mechanical strength of PVDF membrane enables multiple reprobing of blots

➤ Naturally hydrophobic support is ideal for western blotting because it ensures high signal with low background

➤ Available in multiple formats:

■ Convenient precut membrane/blot absorbent filter paper sandwiches
■ Packs of precut membrane-only sheets
■ Economical rolls

➤ Transferring proteins from SDS-PAGE gels to membrane

➤ Immunoblotting target proteins

➤ Detection by western blotting chemiluminescent or colorimetric methods

RB-800 Transfer Membrane PVDF 0.45µm (3cm x 8.5cm) Pk10

RB-900 Transfer Membrane PVDF 0.2µm (7cm x 8.4cm) Pk10

RB-1000 Transfer Membrane PVDF 0.45µm (7cm x 8.4cm) Pk10

Blot Paper

Pre-cut blotting papers are ideal for use in transfer sandwiches and cassettes when proteins are transferred electrophoretically from a gel to a nitrocellulose, PVDF, or other membrane

Features

➤ 3 x 8.5 cm
➤ 7 x 8.5 cm

➤ Thick (about 2.4mm) – for Trans-Blot® SD semi-dry or Trans-Blot transfer cells

➤ Thin (about 0.35mm) – for transfer of thick gels using wet blotting methods

Convenient – pre-cut; works with most mini gel transfer assemblies

Tested – performs consistently without artifacts or background

RB-100 Blot Paper-Wet (3cm x 8.5cm) Pk100

RB-200 Blot Paper-Semi Dry (3cm x 8.5cm) Pk50

RB-300 Blot Paper-Wet (7cm x 8.5cm) Pk100

nUView Workflow

nUView Tris-Glycine UV reactive Precast Gel (NB)(Bio-Rad)

10 gels/box

Gel Size: 10 cm x 8.5 cm (fits Mini-Protean Tetra Cells Tanks)

> for more information <

Conversion Chart:

Mini-PROTEAN®TGX™ and Mini-PROTEAN®TGX Stain-Free™ PrecastGels to nUView Tris-Glycine™ Precast Gel

Product	NB series
Well Number (Vol)	10 (50 µL) 12 (30 µL) 17 (20 µL)
Storage condition/Shelf life	4 °C for 24 Months RT for 6 Months
Recommended sample buffer	TruSep Sample Buffer
Recommended running buffer	Tris-glycine SDS
Available % Acrylamide	8%, 10%, 12%, 8-16%,4-20%
Separation Range	8% (200-45 kDa) 10% (200-10 kDa) 12% (200-21 kDa) 8-16% (200-10 kDa) 4-20% (200-6.5 kDa)
Compatible Tanks	Mini-Protean (tetra and dodeca)
Run Time	35 min
Recommended Voltage	250 V
UV Visualization	in 2 minutes
SDS in gel	No
Cassette Size: (Width x Height x Thickness) cm	10 x 8.5 x 0.5
Gel Size: (Width x Height x Thickness) cm	8 x 7.3 x 0.1

nUView Tris-Glycine UV reactive Precast Gel (NN)(Novex/Invitrogen)

10 gels/box

Gel Size: 10 cm x 10 cm (fits Thermofisher mini-cell tanks)

> for more information <

Conversion Chart:

Bolt® Bis-Tris Plus™ Precast Gels to nUView Tris-Glycine™ Precast Gels
Novex™ Tris-Glycine Wedge Well Precast Gels to nUView Tris-Glycine™ Precast Gels
NuPAGE™ Bis-Tris Precast Gels to nUView Tris-Glycine™ Precast Gels

Product	NN Series
Well Number (Vol)	10 (50 µL) 12 (30 µL) 17 (20 µL)
Storage condition/Shelf life	4 °C for 24 Months RT for 6 Months
Recommended sample buffer	TruSep Sample Buffer
Recommended running buffer	Tris-glycine SDS
Available % Acrylamide	8%, 10%, 12%, 8-16%,4-20%
Separation Range	8% (200-45 kDa) 10% (200-10 kDa) 12% (200-21 kDa) 8-16% (200-10 kDa) 4-20% (200-6.5 kDa)
Compatible Tanks	XCell Surelock Mini
Run Time	50 min
Recommended Voltage	250 V
UV Visualization	in 2 minutes
SDS in gel	No
Cassette Size: (Width x Height x Thickness) cm	10 x 10 x 0.7
Gel Size: (Width x Height x Thickness) cm	8 x 8.8 x 0.1

nUView Tris-Glycine UV reactive Precast Gel (NG)(Cytiva/Hoefer)

10 gels/box

Gel Size: 10 cm x 8 cm (fits Cytiva/Hoefer mini vertical tanks)

Name Change
To improve the consistency of our catalog numbers we have decided to rename our NG precast gels.

NG21 -> NG10
NG11 -> NG12
NG31 -> NG15
* this means NG21-008 is now NG10-008 - the first two numbers represents the number of wells while the last three numbers represents the acrylamide %

> for more information <

Product	NG Series
Well Number (Vol)	10 (50 µL) 12 (30 µL) 15 (25 µL)
Storage condition/Shelf life	4 °C for 24 Months RT for 6 Months
Recommended sample buffer	TruSep Sample Buffer
Recommended running buffer	Tris-glycine SDS
Available % Acrylamide	8%, 10%, 12%, 8-16%, 4-20%
Separation Range	8% (200-45 kDa) 10% (200-10 kDa) 12% (200-21 kDa) 8-16% (200-10 kDa) 4-20% (200-6.5 kDa)
Compatible Tanks	other 10cm Tanks
Run Time	30 min
Recommended Voltage	250 V
UV Visualization	in 2 minutes
SDS in gel	No
Cassette Size: (Width x Height x Thickness) cm	10 x 8 x 0.5
Gel Size: (Width x Height x Thickness) cm	8 x 6.8 x 0.1