Features and Benefits

• Compatible with all standard immunoblotting and nucleic acid detection methods
• Provides superior binding capacity without background interference
• Pore size produces excellent signal-to-noise blotting results
• Available in three formats:
  • Convenient precut 0.45 µm/0.2 µm membrane/blot absorbent filter paper sandwiches
  • Packs of precut membrane-only sheets
  • Economical rolls

Applications

• Transfer of protein from SDS-PAGE gels to membrane in preparation for western blotting
• Transfer of a wide range of protein molecular weights and nucleic acids >500 bp (0.45 µm) / <500 bp (0.2 µm)
• Transfer of nucleic acids in preparation for northern or Southern blotting
• Immunodetection of target proteins and probe hybridization of nucleic acids

Pore size:

0.45 µm --- most blotting applications

0.20 µm --- lower molecular weights

10-15 pieces per package
New size: 30cm x 3.5m/ roll available

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NuSep PVDF Membrane is ideal for chemiluminescent and colorimetric western blotting: it retains target protein very strongly during protein transfer but also reduces nonspecific protein binding that can obscure high-sensitivity detection.

Features and Benefits

• Mechanical strength of PVDF membrane enables multiple reprobing of blots
• Naturally hydrophobic support is ideal for western blotting because it ensures high signal with low background
• Available in three formats:
  • Convenient precut membrane/blot absorbent filter paper sandwiches
  • Packs of precut membrane-only sheets
  • Economical rolls

Applications

• Transferring proteins from SDS-PAGE gels to membrane
• Immunoblotting target proteins
• Detection by western blotting chemiluminescent or colorimetric methods

Advantages

• High binding affinity
• Low background
• Ready-to-use pre-cut membrane/filter paper sandwiches

Two pore sizes available:

0.45 µm --- most blotting applications
0.20 µm --- lower molecular weights

10 pieces per package

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• Specially designed to fit nUView Tris-Glycine Precast Gels 
• Loading samples has never been easier
• Reduces the risk of bursting and distortion of the gel wells 

Quantity: 100 Count

Available in different thicknesses:

• Thick (about 2.4mm) - for Trans-Blot^® SD semi-dry or Trans-Blot transfer cells (50 sheets/pack)
• Thin (about 0.35mm) - for transfer of thick gels using wet blotting methods (100 sheets/pack)

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NuSep’s Snap Cap Spin Columns are convenient tools for manipulating small volumes of affinity supports (20 to 500 µL) for protein purification.
(50/pk)

Features of Snap Cap Spin Columns:

• Formulation: polypropylene
• Column volume: 1 mL
• Resin volume: 20 to 500µL
• Filter type: Polyethylene filter, ~30 µm pore size
• Cap: Attached snap-cap (no cap on collection tube); press-on bottom caps

Simply add the affinity resin and sample to one of the spin columns, then use a microcentrifuge to efficiently wash away contaminants and elute your purified sample without losing any resin in the process. Spin columns allow you to affinity purify more protein in less time.

Applications:
• Affinity purification or affinity chromatography
• Immunodepletion
• Immunoprecipitation (IP)
• Co-immunoprecipitation (co-IP)