Transfer Membranes RSS

Infographic, Lab and Research Generality, Life Sciences, Protein Transfer, Transfer Membranes -

Transfer Basics Protein Transfer on to a Membrane Protein transfer is a vital step in western blot analysis which involves the transfer of proteins separated in a gel by electrophoresis to a solid support matrix. Immobilizing the protein to a solid support matrix facilitates the detection of specific proteins using antibodies directed against the protein(s) of interest. Typical solid matrices are membrane sheets of nitrocellulose and PVDF. Blotting After separating the protein mixture, it is transferred to a membrane. The transfer is done using an electric field oriented perpendicular to the surface of the gel, causing proteins to move out...

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